A Research Paper published in "Avicenna Journal of Medical Biotechnology" , tilted "Regulation Studies of Telomerase Gene in Cancer Cells by Lentinan" by K.Sreenivasulu, Asoc. Professor, BT Department.
A Research Paper published in "Avicenna Journal of Medical Biotechnology, Vol. 2, No. 4, October- December 2010" , tilted "Regulation Studies of Telomerase Gene in Cancer Cells by Lentinan" by K.Sreenivasulu, Asoc. Professor, BT Department.
Lentinan a polysaccharide from medicinal mushroom i.e Lentinus, has been known to have anticancer properties. Telomerase activity is not observed in normal healthy cells, whereas in cancerous cells telomerase expression is high. Telomerase represents a promising cancer therapeutic target. We investigated the inhibitory effect of lentinan on telomerase reverse transcriptase gene (hTERT) which is essential for telomerase activity. To assess the transcriptional effect, DLD -1 cancer cells were cultured in the presence of various concentrations of lentinan. TRAPassay, RT-PCR analysis were performed to find telomerase activity and hTERTgene expression respectively. Since C-myc is known to regulate hTERT, expression of C-myc was also determined. Culturing cells with lentinan resulted in down regulation of hTERT and C-myc expression.These results indicate that lentinan inhibits telomerase activity by down regulating hTERT expression via suppression of C-myc in cancer cells.
Keywords: C-myc gene, Gene expression, hTERT, Lentinan, Telomerase